A cross-sectional study was conducted from December 2020 to January 2021 to determine the species diversity and associated risk factors of ticks on cattle in the study site Haramaya University, CVM, Veterinary clinic and beef cattle farm, Eastern, Ethiopia. During the study period, a total of 50 cattle was considered and examined for presence of ticks and associate risk factors. Ticks were identified to species level by using morphological identification keys under a stereomicroscope. A total of 54 adult ticks were collected and identified to their genera and species level. Two genera and three species were recorded, in which two species each belong to the genus Amblyomma, one species belong to the sub genus Rhipicephalus (Boophilus). Of all the total ticks collected, sub genus Rhipicephalus (Boophilus) and Amblyomma constituted, 20 %, % and 16 % respectively. The adult tick species identified were Boophilus decoloratus (100%) Amblyomma variegatum (62.5 %) and Amblyomma cohaerens (37.5 %). The average burden of adult tick infestation was 3.4 ticks per animal. In this study, poor body conditioned cattle has significantly (P=0.000) higher prevalence of tick infestation during the study period. Therefore, to reduce and avoid losses incurred by ticks infestation, cost effective tick control programs should be formulated and implemented in the study area incorporated with selection of tick resistant breeds of cattle, good management systems and participating and awareness creation of livestock owners regarding tick control and prevention methods.